Figure 2.

Leishmania infantum extracellular products promote a dose-dependent differential cell recruitment. Relative number of total cells (a), eosinophils, neutrophils, macrophages/monocytes and dendritic cells (normalized against the PBS recruitment) (b) and relative cellular abundance (c) in mice pre-formed air pouches 6 h after the injection of PBS (crossed dots), LPS (dark grey dots), 10⁶, 10⁷ and 10⁸ L. infantum promastigotes (black dots) or the EXO (medium grey dots), VDE (light grey dots) and EVs (white dots) produced by the same number of parasites (e⁶, e⁷ and e⁸). Cells were quantified using a Neubauer chamber and the phenotype analysed by flow cytometry. Each dot represents an animal. Bars represent the average (± SEM) obtained from the animals studied in three different experiments. One-sample t-test was used to assess statistical significances comparing to the PBS control group. Significant differences are highlighted in the figure: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.