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. 2018 Jun 7;2(6):351–361. doi: 10.1002/jbm4.10056

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© 2018 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Localization of chondrocyte and perichondrium marker expression by in situ hybridization. (A) Masson's trichrome staining of day 3 postnatal rat proximal tibia including perichondrium layers (cambium (C) and fibrous layer (F)), growth plate cartilage (GP), and metaphyseal bone (MB), as well as representative chondrocyte pellets after 21 days of culture. (B) mRNA expression of chondrogenic (Col2a1 and Col10a1) markers and a cambium‐specific perichondrium marker (Lgals1) in postnatal day 3 (d3) rat proximal tibia and chondrocyte pellet cultures on day 21(d21). The magnification of individual pictures taken is mentioned, with the viewing fields for rat proximal tibia or chondrocyte pellet sections kept the same for histology and in situ hybridization examination. GP = growth plate cartilage; MB = metaphyseal bone; C = cambium (inner) layer; F = fibrous (outer) layer of perichondrium; Col2a1 = collagen type II alpha 1; Col10a1 = collagen type X alpha 1; Lgals1 = l‐galectin.