Figure 2.

Acute and chronic SD-OCT changes of the I307N Rho mouse. Eight- to 12-week-old I307N Rho mice were exposed to 30 minutes of 20,000 lux of light. SD-OCT was performed before or 1, 3, 5, 8, 15, or 30 days thereafter (n = 8 per group, except for day 15, where n = 7). The data do not represent a time-course of changes for a single mouse since repeated administration of anesthesia would be harmful, and mice were euthanized for postmortem analysis. Mice in the day 5 group were an exception and were re-imaged for the day 15 or 30 groups. (A) A spectrum of severity of retinal degeneration was observed in the I307N Rho mouse challenged with the same light damage protocol. Provided are representative SD-OCT B-scans captured at each time point corresponding to mice experiencing either mild or severe retinal degeneration. (B) The total retinal thickness was measured as the distance in micrometers (μm) from the NFL to the RPE in the SD-OCT B-scans. Measurements were performed in the superior, inferior, nasal, and temporal quadrants of the retina. The bar graph represents the mean ± SEM. Two-way ANOVA followed by multiple comparisons tests was utilized to determine statistical significance. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.