Figure 5. HIV replication kinetics in the BLTS humanized mouse model.

(A) Kinetics of HIV-1 replication (HIV RNA genome copies per ml, HIV GE copies per ml) in the blood following inoculation at 1 × 10⁵ IU per mouse in BLTS humanized mice (n = 4 per group). (B) Kinetics of HIV-1 replication (HIV RNA genome copies per ml, HIV GE copies per ml) in the blood of chronic HIV-infected BLTS humanized mice following intervention with triple antiretroviral drug therapy (ART) (n = 6 per group) and subsequent cessation after 4 weeks of treatment (n = 3 per group); viral load was allowed to rebound over a period of 8 weeks. (C) Chronic HIV infection in BLTS humanized mice was allowed to develop over a period of 8 weeks, and animals were subsequently treated with ART for 4 weeks, at which point replication-competent HIV in human resting CD4⁺ T cells from the human spleen organoid of individual mice were analyzed (n = 2) using the TZA method, with CD4⁺ T cells activated using CD3/CD28 stimulation; age-matched mock-inoculated BLTS humanized mouse served as control (ND abbreviates not detectable). Data is presented as mean values ± SEM.