Figure 5. Lipin-1 deficiency reduces DSS-induced inflammation in colon.

WT and lipin-1–deficient (fld) animals were treated with DSS as indicated in the Methods. (A–D) Analysis by quantitative real-time PCR of the mRNA content for the indicated molecules in colon tissues. The amount of each mRNA in the colons of untreated mice was given an arbitrary value of 1 (n ≥ 3). (E–I) Studies were performed at day 8 after treatment initiation. (E) Colon homogenates were used for cytokine quantification using specific ELISAs as indicated (n ≥ 3). (F) Histological evaluation of inflammatory cell infiltration into colonic tissues (n = 4). In A–F data represent the mean ± SEM. (G–I) Lamina propria cells were analyzed by flow cytometry. (G) Total leukocytes are shown as CD45⁺ cells (n = 4). (H and I) CD45⁺ cells were gated and total CD3⁺ and CD4⁺ cells (H) or total CD11b⁺ and F4/80⁺ myeloid cells (I) are shown (n = 4). *P < 0.05; **P < 0.01; ***P < 0.001 by Student’s t test.