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. 2018 Sep 20;3(18):e97506. doi: 10.1172/jci.insight.97506

Figure 6. Lipin-1 expression level in macrophages determines colitis severity.

Figure 6

(A and B) Macrophages isolated from DSS-treated WT and lipin-1–deficient (fld) mouse colons were used for quantification of the indicated cytokines by specific ELISAs (n = 3) (A), or for quantification of PAP-1 activity, as indicated in the Methods (n = 4–6) (B). Data represent the mean ± SEM. (C–F) WT and fld animals were subjected to macrophage transference during the treatment with DSS, as mentioned in the Methods. Weight change (C), DAI score (D), total histology score (E), and images of H&E-stained colon sections (F) 8 days after DSS treatment initiation are shown (n = 4–5 animals/group). In E, data represent the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 by Student’s t test (A and B) or 1-way ANOVA with Tukey’s post hoc test (C–E). In F, original magnification was ×10.