Skip to main content
. 2018 Sep 20;3(18):e120493. doi: 10.1172/jci.insight.120493

Figure 3. FKRP-mutant zebrafish have significantly impaired vascularization and increased unfolded protein response (UPR) transcripts.

Figure 3

(A) Reduced vasculature in the fkrp–/– zebrafish compared with sibling controls. The Tg(actc1b:RFP) (labels myofibers red) and flk1-GFP (labels vasculature green) transgenic alleles were bred onto the fkrp-mutant zebrafish strain background. Scale bars: 100 μm. (B) Intersegmental vessel length (ISV) is quantified in the fkrp–/– zebrafish compared to sibling controls. n = 5 zebrafish per cohort. (C) Real-time quantitative PCR of expression levels of UPR genes (atf4a, atf4b, atf6, atf6b, hspa5/BiP, ern1/IRE1, eif2ak3/PERK, xbp1) were measured and normalized to β-actin as housekeeping control. For all experiments, 5 zebrafish (n = 5) per cohort were used. *P < 0.05 by 2-tailed Student’s t test.