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. 2018 Sep 20;3(18):e121886. doi: 10.1172/jci.insight.121886

Figure 7. IFN-γ mediates enteroid damage induced by splenocyte activation.

Figure 7

(A) WT or IFN-γ receptor–KO (IFN-γRKO, KO) enteroids in coculture with WT splenocytes with or without activation using anti-CD3/anti-CD28 beads. (B) Damage scores of WT or KO enteroids in coculture with control or activated WT splenocytes. Data are representative of 2 independent experiments and shown as mean ± SEM, with n = 100 enteroids per group. (C) qRT-PCR analyses to quantify lineage-specific and apoptosis marker mRNAs in WT or IFN-γRKO enteroids in coculture with control or activated WT splenocytes for 4 days. Data are representative of 2 independent experiments and shown as mean ± SEM (n = 5 independent wells). Dunnett’s multiple comparisons test was used to compare each group with the WT control group. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: 200 μm.