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. 2018 Sep 20;3(18):e120179. doi: 10.1172/jci.insight.120179

Figure 2. Generation of IFI16 cytoplasmic filaments in response to dsDNA transfection in epithelial cells in vitro.

Figure 2

(A–C) Human salivary gland (HSG) cells were treated with recombinant IFNα prior to transfection with empty plasmid DNA. Cells were then fixed, permeabilized, and stained with anti-IFI16 antibody (green) and DAPI (blue). Representative confocal images at 100× magnification are shown. Camera settings were held constant between experiments. (D) Keratinocyte cultures were transfected with Rhodamine labeled Poly(dA:dT) (red) and then stained for IFI16 (green) and counterstained with DAPI (blue). A 3-dimensional rendering of a Z-stack series is shown. (E) DNA titration was performed in HSG cultures using increasing concentrations of plasmid DNA, followed by staining with anti-IFI16 (green) and DAPI (blue). Cells with cytoplasmic IFI16 were counted in 4 fields imaged at 40×. Mean values with standard deviation are indicated. *P < 0.05; ***P < 0.0005; ****P < 0.0001 as assessed by the Mann-Whitney U test. Scale bars: 5 μM. Data are representative of results of 3 experiments.