Figure 4. IFI16 protein filaments persist after nuclease treatment.
(A) Recombinant IFI16 was combined with dsDNA600 and then treated with micrococcal nuclease and imaged by negative stain electron microscopy. (B and C) Replicate samples were analyzed by agarose gel electrophoresis and stained with SYBR green to confirm effectiveness of nuclease treatment (B) and by SDS-PAGE and Western blotting for IFI16 (C). Scale bars: 100 nM.