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. 2018 Sep 20;3(18):e120179. doi: 10.1172/jci.insight.120179

Figure 5. Autoantibody Immunoprecipitation of IFI16 is enhanced in the presence of dsDNA.

Figure 5

(A and B) Serum samples from 56 Sjögren’s Syndrome patients positive for IFI16 antibodies by ELISA were used to immunoprecipitate IFI16 in the absence or presence of 150 bp dsDNA in 10:1 IFI16/dsDNA molar ratio. Immunoprecipitation products were electrophoresed and Western Blotted with a commercial IFI16 antibody. (A) Representative data from immunoprecipitations performed with 16 patients’ sera and an N-terminal mouse monoclonal antibody (MM) are shown. Sera 1 and 4 were negative for anti-IFI16 antibodies by ELISA and were included as negative controls. Input lanes contain one-fourth protein used in each serum immunoprecipitation. (B) All 56 immunoprecipitations were quantified by densitometry. For each, the ratio of immunoprecipitated IFI16 detected in the presence versus absence of dsDNA was calculated. The dotted line indicates a ratio of 1, denoting unchanged immunoprecipitation with or without dsDNA. Median with interquartile range are indicated with solid lines.