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. 2018 Sep 20;3(18):e120179. doi: 10.1172/jci.insight.120179

Figure 6. C-terminal specificity of SS sera with property of IFI16-DNA binding.

Figure 6

(A) Cytoplasmic IFI16 filaments were induced in human salivary gland (HSG) cells and stained with commercial antibodies recognizing C-terminal (green) and N-terminal (red) epitopes. Both commercial antibodies recognize nuclear IFI16 (asterisk), but only the C-terminal antibody binds the cytoplasmic filament (arrow). (B) SS sera with or without the property of DNA-enhanced IFI16 immunoprecipitation were used to immunoprecipitate full-length IFI16 (FL) or an N-terminal fragment (IFI16ΔCT) lacking residues 597–729 at the C-terminus; data from Figure 5B specific to these 10 sera are shown. Commercial antibodies specific for the N-terminus (anti-NT) and C-terminus (anti-CT) were included. (C) Western blots were performed with anti-N terminal antibody. Blots were scanned by densitometry and the ratio of immunoprecipitated IFI16ΔCT/IFI16 FL in each was quantitated (Supplemental Table 2). Representative data obtained with 2 patient sera and the commercial antibodies is shown. Scale bar: 10 μM. Data are representative of results of 2 experiments.