Fig. 1.
CtBP knockdown increases micronuclei formation in glycolytic cells. (a) The effect of individual and combined knockdown of CtBP1 and CtBP2 on micronuclei formation in MCF-7 cells. CtBP siRNA targeting a common region in both CtBP1 and CtBP2 has been described previously, as have siRNAs targeting individual CtBP1 and CtBP2 mRNAs.32 Effectiveness of siRNA knockdown is shown in Fig. S1.† (b) The effect of upregulated glycolysis on the requirement for CtBPs for the maintenance of mitotic fidelity. Cells cultured in medium containing either glucose or fructose were transfected with the indicated siRNA.