Table 2. Comparison between the TCRs detected by the single cell RNA-Seq and the bulk RNA-Seq of the CD8+ T cells from the MC38 tumor and the mouse spleen.

Tissue source	Locus	measurement	single cell only	overlap	cell pool only
MC38 tumor	TCRα	#unique TCRs	17	16	250
		#cells	16 (1 biallelic)a	47 (6 biallelic)	⎯
		%reads	⎯	73.2%	26.8%
	TCRβ	#unique TCRs	28	16	618
		#cells	27 (1 biallelic)	51	⎯
		%reads	⎯	70.8%	29.2%
Spleen	TCRα	#unique TCRs	61	1	669
		#cells	58 (3 biallelic)	1	⎯
		%reads	⎯	1.3%	98.7%
	TCRβ	#unique TCRs	82	1	2355
		#cells	78 (4 biallelic)	1	⎯
		%reads	⎯	0.03%	99.97%

^a One cell among the 16 cells is biallelic at the TCRα locus. I.e. It has two unique TCRαs. The annotation is applicable to other similar expressions.