Fig 2. rAAV8 capsid degradation by v-CATH studied with anti-VP polyclonal antibody.

rAAV8 vectors encoding the γSGC transgene were produced in an unmodified bacmid system in the presence or the absence of E64 protease inhibitor and purified using immuno-affinity chromatography. Purified vectors were loaded on SDS-PAGE stained with Coomassie blue (left panel). The corresponding Western blot is shown on the right panel.