Fig 7. Supplementary truncated VP1 band is a product of translation and not a degradation product.

Origin of supplementary truncated VP1 protein band (signaled on the figure by *) was studied by Western blot using anti-VP B1 antibody. (1) AAV2 WT. 5 x 10⁹ vg of AAV2 WT produced by transfection of HEK 293 cells. (2) Empty AAV2. AAV2 empty capsids produced with baculovirus deleted of chiA/v-cath genes, with the cap2 gene codon optimized. (3) Empty AAV2 with silenced VP1 expression. AAV2 empty capsids produced with baculovirus deleted of chiA/v-cath genes, with cap gene codon optimized, including silencing of VP1 alternative start codon (ACG → ACT).