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. 2018 Nov 5;14(11):e1007469. doi: 10.1371/journal.pgen.1007469

Fig 5. HDG3 is imprinted in Cvi HDG3 IR lines.

Fig 5

(A) HDG3 in situ hybridization for indicated genotypes. Arrowheads indicate regions of in situ signal. Right panels shows magnification of chalazal region. Scale bars, 50 μm. (B) RT-qPCR of relative HDG3 transcript abundance in F1 endosperm at 6–7 DAP. Dashed line separates experiments done at different times. Left, avg of 3 technical replicates. Right, avg of biological duplicates. Bars show upper and lower range. (C) % of HDG3 from Cvi allele in endosperm by TaqMan RT-qPCR assay.