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. 2018 Nov 5;14(11):e1007251. doi: 10.1371/journal.ppat.1007251

Fig 5. Accumulation of IgT+ B cells in the olfactory organ of trout infected with Ich.

Fig 5

DIC images of immunofluorescence staining on trout nasal paraffinic sections from uninfected fish (A), 28 days infected fish (B) and survivor fish (C), stained for IgT (green) and IgM (red); nuclei are stained with DAPI (blue). (D) Enlarged images of the areas outlined in c are showing some IgT+ B cells possibly secreting IgT (white arrowhead) (isotype-matched control antibody staining, S1B Fig). NC, nasal cavity; OE, olfactory epithelium; LP, lamina propria. Scale bar, 20 μm. Data are representative of at least three different independent experiments (n = 8 per group).