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. 2018 Oct 24;17(19-20):2335–2348. doi: 10.1080/15384101.2018.1526598

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Figure 8. — P27 affects the formation of CDK6/CCND1 complex. (a) qPCR was used to detect the expression of CDK6 and CCND1 after regulating the expression of P27 in A549 cells. (b) Co-immunoprecipitation and Western blotting were combined to examine the effects of p27 on formation of the CDK6/CCND1 complex after regulating the expression of P27 in A549 cells. (c) The distribution of CDK6 and CCND1 was detected by immunofluorescence after regulating the expression of p27 in A549 cells. (d) Phosphorylation of Rb was detected when the expression of p27 was changed in A549 and HTB182 cells.