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. 2018 Oct 22;17(19-20):2349–2359. doi: 10.1080/15384101.2018.1526599

Figure 4.

Figure 4.

miR-200a is a direct downstream target of circRNA 101368 (a) HepG2 and HCCLM3 cell lines were transfected with miR-200a mimics or miR-200a inhibitor to achieve miR-200a expression, as confirmed using real-time PCR; (b) transfected HCC cell lines were examined for circRNA 101368 expression. (c) HepG2 and HCCLM3 cell lines were transfected with si-circRNA 101368 and examined for miR-200a expression. (d) The miR-200a binding site in circRNA 101368 predicted by lncTar was shown. A wild-type and a mutant-type circRNA 101368 luciferase reporter vector were constructed, containing wild-type or mutant-type miR-200a binding site, respectively. (e) HEK293 cells were co-transfected with the above luciferase reporter vectors and miR-200a mimics/inhibitor and examined for luciferase activity. (f-g) Association of circRNA 101368, miR-200a and HMGB1 with AGO2. Detection of AGO2 and IgG using Immunoblotting and detection of circRNA 101368, miR-200a and HMGB1 using real-time PCR.