Table 1. CryoEM data collection, refinement, and model validation statistics.
| Parameter | Value |
|---|---|
| Data Collection | |
| No. of Micrographs | 1993 |
| No. of Particles | 134,443 |
| Pixel size, Å | 1.36 |
| Defocus range, μM | 2.0–3.5 |
| Voltage, kV | 300 |
| Dose Rate, counts/pix/sec | 8 |
| Electron dose, e-/Å2 | 43 |
| Refinement | |
| Resolution, Å | 3.8 |
| Map-sharpening B factor, Å2 | −230 |
| Model validation (3 Fab structure) | |
| Favored rotamers, % | 98.36% |
| Poor rotamers, % | 0.30% |
| Ramachandran outliers, % | 0.13% |
| Clash Score | 0.99 |
| Molprobity score EM ringer score |
1.02 1.97 |