Table 1. Overview of fermentation experiments and model fitting results (RMSE: root mean square error).
Means and standard deviations are reported across biological replicates.
| Strains | Number of biological replicates | Added energy source and/or co-substrate | Consumption (-)/production (+) of metabolites in mM | Carbon recovery (%) | O/R balance | Selected for parame-terization 1 | RMSE parame-terization 1 | Selected for parame-terization 2 | RMSE parame-terization 2 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fructose | Acetate | Butyrate | Formate | Lactate | H2 | CO2 | |||||||||
| Monoculture fermentations | |||||||||||||||
|
R. intestinalis
L1-82 |
4 | Fructose/acetate | −48.8 ± 0.9 | −20.6 ± 2.4 | 58.5 ± 3.9 | 6.8 ± 1.2 | 3.9 ± 1.1 | 72.2 ± 4.9 | 84.0 ± 0.8 | 105 ± 14.3 | 0.86 ± 0.2 | Yes | 0.17 ± 0.04 | No | 0.83 ± 0.13 |
| F. prausnitzii A2-165 | 3 | Fructose/acetate | −23.1 ± 1.1 | −13.5 ± 0.8 | 29.8 ± 0.2 | 22.7 ± 0.9 | 1.8 ± 0.2 | 0.2 ± 0.2 | 19.3 ± 1.6 | 100.9 ± 3.4 | 1.05 ± 0.09 | Yes | 0.15 ± 0.08 | Yes | 0.15 ± 0.08 |
| B. hydrogenotrophica S5a33 | 3 | Fructose/formate | −19.0 ± 5.6 | 23.0 ± 6.6 | 0.0 ± 0.0 | −36.1 ± 1.8 | 6.0 ± 1.2 | 31.5 ± 7.7 | 26.0 ± 5.3 | 60.0 ± 2.1 | 0.76 ± 0.07 | Yes | 1.43 ± 0.27 | No | 3.53 ± 1.04 |
| Bi-culture fermentations | |||||||||||||||
|
R. intestinalis
L1−82/F. prausnitzii A2-165 |
2 | Fructose/acetate | −47.7 ± 2.2 | −18.1 ± 2.7 | 56.3 ± 4.5 | 7.6 ± 2.7 | 4.3 ± 3.3 | 114 ± 16 | 85.7 ± 4.1 | 102.8 ± 0.3 | 0.79 ± 0 | No | 0.77 ± 0.06 | No | 0.68 ± 0.03 |
|
R. intestinalis
L1−82/B. hydrogenotrophica S5a33 |
2 | Fructose/acetate | −46.5 ± 1.5 | −7.5 ± 1.9 | 53.5 ± 4.7 | −0.5 ± 0.8 | 2.2 ± 0.3 | 53.0 ± 3.0 | 74.9 ± 4.1 | 100.2 ± 0.6 | 0.94 ± 0.12 | No | 0.78 ± 0.36 | Yes | 0.3 ± 0.04 |
|
R. intestinalis
L1−82/B. hydrogenotrophica S5a33 |
2 | Fructose | −48.0 ± 0.2 | 44.9 ± 5.4 | 27.9 ± 3.0 | −1.0 ± 0.0 | 5.4 ± 2.4 | 33.1 ± 3.2 | 47.4 ± 3.9 | 91.6 ± 1.1 | 1.05 ± 0.06 | No | 0.9 ± 0.17 | No | 0.32 ± 0.04 |
|
F. prausnitzii
A2−165/B. hydrogenotrophica S5a33 |
2 | Fructose/acetate | −49.3 ± 1.0 | 41.4 ± 11.7 | 30.7 ± 1.8 | −1.2 ± 0.0 | 3.9 ± 1.6 | 56.2 ± 37.1 | 54.8 ± 33.3 | 91.6 ± 9.4 | 0.88 ± 0.25 | No | 0.6 ± 0.14 | Yes | 0.26 ± 0.13 |
|
F. prausnitzii
A2−165/B. hydrogenotrophica S5a33 |
1 | Fructose | −47.0 | 62.5 | 25.5 | −1.1 | 4.5 | 62.9 | 63.6 | 107.4 | 1.11 | No | 0.63 | No | 0.46 |
| Tri-culture fermentations | |||||||||||||||
|
R. intestinalis
L1−82/F. prausnitzii A2−165/B. hydrogenotrophica S5a33 |
6 | Fructose | −48.9 ± 1.3 | 38.4 ± 16.9 | 32.5 ± 5.5 | −1.2 ± 0.1 | 7.4 ± 1.7 | 61.9 ± 3.2 | 57.1 ± 9.2 | 97.0 ± 3.1 | 0.84 ± 0.09 | No | 0.78 ± 0.27 | No | 0.58 ± 0.11 |