Skip to main content
. 2018 Oct 18;3(20):e121900. doi: 10.1172/jci.insight.121900

Figure 1. GJA1-20k is upregulated with acute cardiac I/R injury.

Figure 1

(A) Schematic of the protocol used for I/R injury in Langendorff-perfused mouse hearts. After a 20-minute stabilization period, mouse hearts were subjected to continuous perfusion for 90 minutes as a control, or subjected to 30 minutes of ischemia followed by 60 minutes of reperfusion for I/R injury. (B) Western blot of heart tissue lysates after either I/R injury or control perfusion. The blots are probed with an antibody for Cx43 C-terminus and an anti-actin antibody. (C) Protein expression for GJA1-20k is normalized to actin and shown as fold change after I/R injury in the quantified graphs. (D) Western blot showing Cav1.2 protein levels in the control perfused hearts and after I/R injury. (E) Cav1.2 expression is normalized to actin and shown as fold change after I/R injury in the quantified graph. The number of hearts examined in C and E is shown on the graphs, and data are presented as mean fold change ± SEM. **P < 0.01, Mann-Whitney U test.