Figure 5. IAV reduces ENaC and CFTR short-circuit currents across intact normal human bronchial epithelial cell monolayers.

NHBEs were grown at an air-liquid interface and infected with A/California/07/2009 H1N1 IAV at a MOI of 3 for (A) 24, (B) 48, and (C) 72 hours, at which point they were mounted in Ussing chambers for the measurement of short-circuit currents (I_sc) and transepithelial resistance. Amiloride (ENaC) and forskolin-sensitive currents inhibited by the specific CFTR inhibitor CFTR_inh172 were significantly reduced by IAV infection. Amilo., amiloride trough; Forsk Pk., forskolin peak; Forsk. Plt., forskolin plateau. Characteristic records (A–C) and scatter plots (n = 6 filters per groups; mean ± 1SEM) (D–F). Data were analyzed by 1-way ANOVA and post hoc Tukey test for multiple comparisons. *P < 0.0001 compared with time-matched noninfected controls.