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. 2018 Oct 18;3(20):e123467. doi: 10.1172/jci.insight.123467

Figure 8. IAV decreases specific subunits of ENaC, CFTR, and Na/K-ATPase at the cell surface.

Figure 8

NHBEs were grown at an air-liquid interface and infected with A/California/07/2009 at a MOI of 3. (A) ENaC, CFTR, and Na/K-ATPase protein abundance was measured by Western blotting. For ENaC and CFTR measurements, biotinylated apical membrane proteins were immunoblotted with specific antibodies against ENaC subunits and CFTR, respectively. Blots are representative of 3 separate experiments. (B) Densitometry demonstrates reductions in ENaC subunits, CFTR, and α-Na/K-ATPase subunit, but no difference was observed in the expression of the regulatory β subunit Na/K-ATPase levels. Data were analyzed by 1-way ANOVA and post hoc Tukey test for multiple comparisons. N = 3 per group. *P < 0.001 compared with mock-infected controls.