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. 2018 Oct 18;3(20):e121521. doi: 10.1172/jci.insight.121521

Figure 2. Ent1 and Ent2 inhibition is protective in acute experimental colitis.

Figure 2

Sex-, age-, and weight-matched C57BL/6 WT mice were treated with dipyridamole (combined Ent1 and Ent2) inhibitor, 5 mg/kg, i.p., or vehicle 2–3 times daily from 1 day prior to exposure to DSS. (A) Daily weight measurements were obtained for each group of mice and are displayed as percentage of the body weight average from day 0–3. (B) Following sacrifice, colons were harvested and measured. (C) Mice were administered FITC-dextran by oral gavage (0.6 mg/g at 100 mg/ml) 4 hours prior to sacrifice on day 7. Serum was harvested at sacrifice, and fluorescence measurement was used to determine FITC levels. n = 7 mice/group from 1 independent experiment. (D) Blinded histological analysis of whole colon from each group following DSS exposure. Representative histological sections from whole colon harvested on day 7 after DSS (scale bars: 100 μm; images acquired at ×10). Unless otherwise stated, results of 2 independent experiments are presented as mean ± SEM (n = 4–17 mice/group). Two-way ANOVA with post hoc Bonferroni’s t test was used to determine statistical weight change. Two-way ANOVA with post hoc Tukey’s multiple comparison test was used to determine statistical colon length change. In all other cases, unpaired Student’s t test was used. *P < 0.05.