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. 2018 Oct 18;3(20):e121521. doi: 10.1172/jci.insight.121521

Figure 4. Deletion of Ent2 protects from inflammation and injury in DSS colitis.

Figure 4

Matched Ent2-deficient mice (Ent2–/–) or WT controls (Ent2+/+, mice on a B6/129 background) were exposed to DSS. (A) Weights were obtained for each group of mice and are displayed as percentage of body weight on day 0. (B) Following sacrifice, colons were harvested and measured. (C) Mice were administered FITC-dextran by oral gavage (0.6 mg/g at 80 mg/ml) 4 hours prior to sacrifice on day 7. Serum was harvested at sacrifice, and fluorescence measurement was used to determine FITC levels. n = 5–7 mice/group from 1 independent experiment. (D) Following sacrifice on day 7, whole colonic tissue was snap frozen. Total RNA was extracted and RT-PCR. performed. mRNA transcript levels were calculated relative to β-actin and are expressed as fold change compared with DSS-treated WT mice. Data represent 5–8 mice per group from 1 independent experiment. (E) Representative histological sections from whole colon harvested on day 7 after DSS (scale bars: 100 μm; images acquired at ×10). (F) Histological analysis of whole colon harvested on day 7 after DSS provided by a pathologist blinded to the groups and the study. Unless otherwise stated, there were n = 7–9 mice/group. presented are representative of at least 3 independently performed experiments. Graphs show data as the mean ± SEM. Two-way ANOVA with post hoc Bonferroni’s t test was used to determine statistical weight change; in all other cases, unpaired Student’s t test was used. *P < 0.5, **P < 0.001.