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. 2018 Oct 18;3(20):e121521. doi: 10.1172/jci.insight.121521

Figure 8. Specific pharmacological inhibition of Ent2 ameliorates experimental colitis.

Figure 8

Sex-, age-, and weight-matched C57BL/6 WT mice were treated with soluflazine (Ent2 inhibitor, 7.7 μg/kg, Alzet pump) or vehicle 1 day prior to exposure to DSS. (A) Chemical structure of Ent2 inhibitor (soluflazine). (B) Each group of mice was weighed daily. Weights are presented as percentage of body weight on day 0. (C) Following sacrifice, colons were harvested and measured. (D) Histological analysis of whole colon from each group following DSS. Scores were provided by a pathologist blinded to the groups and the study. Representative histological sections from whole colon harvested on day 7 after DSS (scale bars: 200 μm; images acquired at ×10). Results of 2 independent experiments are presented as mean ± SEM (n = 2 mice/water group and n = 10 mice/DSS group). Two-way ANOVA with post hoc Bonferroni’s t test was used to determine statistical weight change; in all other cases, unpaired Student’s t test was used. *P < 0.05.