(A) Indirect fluorescence ELISA analysis of allergenic extracts. (B) Western blot (WB) analysis of allergenic extracts. Analyses were performed on commercially manufactured allergenic extracts from GREER, HS, ALKB, and AlrgyL using human IgE mAbs to the major allergen Asp f 1. Box-and-whisker plots show the median, first and fourth quartiles, and minimum and maximum values (whiskers).