Human FcεRIα–transgenic mice were sensitized with human Asp f 1–specific IgE mAbs 17A5 and 14H4 or saline. Animals were challenged with 5 μg of recombinant Asp f 1 or the maximum tolerated dose of extract manufactured by GREER, HS, ALKB, or AlrgyL. Each group had 3 mice. Error bars represent the SEM, with temperature differences between control and allergen-challenged animals assessed by paired 2-tailed t test with a calculated P value of less than 0.05 deemed significant and highlighted by an asterisk. rAsp f 1, recombinant Asp f 1.