Figure 4.

Invasiveness of HepG2, clone 3 and clone 5 cells in vitro. A, Representative light microscopy images documenting the invasive potential of HepG2, clone 3 and clone 5 cells after 48, 96, 144 and 192 h of incubation in the spheroid invasion assay (0 h—initial spheroids). Scale—200 μm. B, Time dependent relative invasion of HepG2, clone 3 and clone 5 cells as determined by measuring the area of the spheroids (size of initial spheroids at 0 h was set to 1). Values represent means ± SD and statistical analysis was performed with two‐way ANOVA followed by Dunnett's multiple comparisons test. (HepG2: n = 12, clone 3: n = 11, clone 5: n = 11). **P < 0.01, ***P < 0.001