Figure 4.

IDO expression is up‐regulated in mesenchymal stromal cells (MSC) that home to AKI kidneys, but the increased renal IFNγ following pFUS does not further up‐regulate IDO expression. Kidney homogenates from WT and INFγ‐KO mice that had AKI and received MSC treatment with or without pFUS were assayed for IDO expression by ELISA. For, mice that received MSC without pFUS, greater IDO expression was observed in WT mice compared to INFγ‐KO mice (P < 0.0001). This represents IDO activation through AKI‐expressed INFγ‐KO. However, when WT mice received pFUS to further up‐regulate INFγ, on a statistically insignificant trend for additional IDO expression was observed (P = 0.1). Displayed IDO quantities were background corrected using AKI kidneys from mice that did not receive MSC treatment and normalized to relative MSC quantities present in kidneys with and without pFUS