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. 2018 Sep 25;22(12):6190–6201. doi: 10.1111/jcmm.13906

Figure 3.

Figure 3

FSTL5 suppresses growth of HCC cell lines by inducing apoptosis in vitro. (A) Western blotting showing plasmid transfection efficiency. (B) CCK‐8 assay results showing the growth curve of SMMC7721/Bel7404 cells after transfection with pcDNA3.1(+)‐FSTL5 or pcDNA3.1(+) (n = 5, ***P < 0.001, Student's t test). (C and D) Colony formation assay of SMMC7721/Bel7404 cells after transfection with pcDNA3.1(+)‐FSTL5 or pcDNA3.1(+) (n = 3, ***P < 0.001, Student's t test). (E) Flow cytometry assay to determine cell cycle stages of SMMC7721/Bel7404 cells 72 hours after transfection with pcDNA3.1(+)‐FSTL5 or pcDNA3.1(+) (n = 3, NS, Student's t test). (F and G) Flow cytometry assay for apoptosis of SMMC7721/Bel7404 48 hours after transfection with pcDNA3.1(+)‐FSTL5 or pcDNA3.1(+) (n = 3, ***P < 0.001, Student's t test)