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. 2018 Sep 25;22(12):6190–6201. doi: 10.1111/jcmm.13906

Figure 4.

Figure 4

Knockdown of FSTL5 promotes HCC cell line and LO2 growth in vitro. (A) qPCR showing FSTL5 mRNA expression in SMC7721‐FSTL5 cells after transfection siRNA targeting FSTL5 and siNC (negative control) (n = 4, **P < 0.01, Student's t test; ns, no significant difference). (B) Western blotting analysis of FSTL5 protein expression in SMC7721‐FSTL5 and Bel‐7404‐FSTL5 cells after transfection siRNA targeting FSTL5 and siNC. (C) CCK‐8 assay results showing the growth curve of SMC7721‐FSTL5 and Bel‐7404‐FSTL5 cells after transfection siRNA targeting FSTL5 and siNC (n = 5, **P < 0.01, Student's t test). (D) Western blotting analysis of FSTL5 protein expression in LO2 cells after transfection siRNA targeting FSTL5 and siNC. (E) CCK‐8 assay results showing the growth curve of LO2 cells after transfection siRNA targeting FSTL5 and siNC (n = 5, **P < 0.01, Student's t test)