Figure 2.

Meridianin C does not induce apoptosis of YD‐10B cells. (A) YD‐10B cells were treated with meridianin C (1 μM) or vehicle control (DMSO) for the times indicated. At each time‐point, extra‐nuclear fragmented DNA was extracted, and analysed on a 1.7% agarose gel. The images are representative of three independent experiments. SM, DNA size marker. (B) YD‐10B cells were treated with meridianin C (1 μM) or vehicle control (DMSO) for the times indicated and evaluated for apoptotic subG1 DNA content by flow cytometer. The tables represent the fraction of apoptotic cells. Data are mean ± SEM of three independent experiments. (C) YD‐10B cells were treated with meridianin C (1 μM) or vehicle control (DMSO) for the times designated. At each time‐point, whole cell lysates were prepared, and analysed for procaspase‐9, DR5, PARP cleavage and β‐actin by Western blotting. The images are representative of three independent experiments. (D) YD‐10B cells were treated with meridianin C (1 μM) in the absence or presence of z‐VAD (50 μM), a pan‐caspase inhibitor, for 48 h, and cell survival counted by the trypan blue dye exclusion assay. Data are mean ± SEM of three independent experiments, each performed in triplicate. *P < 0.05 compared to the control at the indicated time. (E) YD‐10B cells were treated with meridianin C (1 μM) in the absence or presence of z‐VAD (50 μM), a pan‐caspase inhibitor, for 48 h. Images were obtained by phase contrast microscopy, 400 X. Each image is representative of three independent experiments