Figure 7.

Nrf2 and p62 co‐IP with Keap1 after HMGB1 siRNA transfection. (A) Co‐IP of Nrf2 and p62 from NHEM lysates. NHEMs transfected with HMGB1 siRNA or scrambled siRNA were stimulated with H₂O₂ (0.5 mM) for 12 hours, and whole‐cell lysates were immunoprecipitated with antibodies specific for Keap1. IgG was used as a control. The precipitated complexes were separately immunoblotted with Nrf2 or p62‐specific antibodies. IP, immunoprecipitation; WB, western blotting. The blots are representative of three experiments with similar results