Figure 2.

The homogeneity analysis by SDS‐PAGE of 8 μg untret‐IgG_mix (lane 1), mog‐IgG_mix (lane 2), dna‐IgG_mix (lane 3) and hist‐IgG_mix (lane 4) under non‐reducing conditions (A, lanes 1‐4); silver staining. The arrow (lane C, A) shows the positions of proteins with known molecular masses. The relative activities (RA, %) of eluate gel fragments in the hydrolysis of DNA (o), MOG (▲and histones (▵) by hist‐IgG_mix were estimated using the extracts of gel fragments (2‐3 mm) (B). Panel C shows the position of IgGs. A complete hydrolysis of all substrates after 24 hours of incubation was taken as 100% (B). The errors of the RAs estimations from two independent experiments did not exceed 7%‐10%. For other details, see “Materials and methods”