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. 2018 Sep 6;22(12):5964–5977. doi: 10.1111/jcmm.13869

Figure 4.

Figure 4

Gentisic acid reduces GATA4 and Sp1 expression in mice with transverse aortic constriction (TAC). Total RNA was isolated from the hearts of mice in the sham, TAC, TAC + gentisic acid, TAC + bisoprolol, and TAC + diltiazem groups. Levels of Sp1 (A) and Gata4 (B) transcripts were determined by RT‐qPCR and normalized to those of Gapdh. Data are means ± SE. C, Representative western blot images showing Sp1 and GATA4 protein levels in the hearts of mice. GAPDH was used as a loading control. The images were cropped from the same blot. D and E, Sp1 and GATA4 protein expression was quantified using densitometry. *P < 0.05, **P < 0.01, and ***P < 0.001; # P < 0.05, ## P < 0.01, and ### P < 0.001 vs the TAC group