Figure 6.

Deguelin inhibits the binding of Bmi1 to the Noxa gene locus in NSCLC cells. A, Schematic representation of the Noxa locus and the location of primers (#1 to #4) used in ChIP experiments and exons are indicated. B‐D, NCI‐H23 (B), NCI‐H1299 (C), and NCI‐H460 (D) cells were treated with DMSO or the indicated concentrations of deguelin for 48 hours and subjected to ChIP assays with an antibody against Bmi1 or normal rabbit IgG. The precipitated DNA fragments were subjected to PCR analysis to test for the presence of sequences corresponding to the Noxa gene locus. Input material (10%) was shown for comparison