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. 2018 Sep 24;22(12):5888–5898. doi: 10.1111/jcmm.13858

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© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Both miR‐181a‐5pand SBP2 regulate the expression of 3 selenoproteins in chondrocytes. (A) Left: The protein expressions of SBP2 and three selenoproteins in C28/I2 cell line transfected with mimic of hsa‐miRNA‐181a‐5p. Right: The protein expressions of SBP2 and three selenoproteins in C28/I2 cell line transfected with inhibitor of hsa‐miRNA‐181a‐5p.(B) The protein expressions of SBP2 and selenoproteins after inhibitor‐181a‐5p infection in Se deficient or Se replenished C28/I2 chondrocytes.(C) The protein expressions of SBP2 and 3 selenoproteins in C28 cells transfected with siRNA‐SBP2. (D) The mRNA expressions of SBP2 and 3 selenoproteins in C28 cells transfected with siRNA‐SBP2.(E) The protein expressions of SBP2 and 3 selenoproteins in C28 cells infected with Ad‐SBP2 plasmid.(F) The mRNA expressions of SBP2 and 3 selenoproteins in C28 cells infected with Ad‐SBP2 plasmid. Data were presented as means ± SEM. *, ** and *** stand for P < 0.05, 0.01 and 0.001, respectively.