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Information of rat primers for real‐time PCR
| Gene | Sequence (5′‐3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| Sbp2 | Forward: AAGGCACCCTAGCTTCCACT | 116 | 60 |
| Reverse: GGTTGATGCCTCCAGTGAGT | |||
| GPx1 | Forward: GGCTCACCCGCTCTTTACCTTC | 168 | 62 |
| Reverse: CGCACTGGAACACCGTCTGG | |||
| GPx4 | Forward: AATTCGCAGCCAAGGACATCG | 168 | 62 |
| Reverse: CCAGGATTCGTAAACCACACTCG | |||
| SelS | Forward: TTCCTGCACGTCACAGTGGG | 115 | 60 |
| Reverse: TTAAAGCCCTCAGTCGCAGG | |||
| GAPDH | Forward: CGGCAAGTTCAACGGCACAG | 148 | 60 |
| Reverse: GAAGACGCCAGTAGACTCCACGAC |
