Figure 10.

Effect of Tp92 on the secretion of cytokines by THP‐1 cells. A and B, Concentrations of secreted cytokines determined by ELISA. The cells were treated with (A) 0, 0.1, 2.5, 5.0, 7.5 or 10.0 μg/mL Tp92 or LPS (1 μg/mL)+Nig(5 μM) for 12 hours or (B) 5.0 μg/mL Tp92 for 0, 4, 8, 12, 24 or 48 hours. *P < 005 compared with (A) PBS or (B) 0 hour. C and D, Effect of the NF‐κB inhibitor QNZ on the secretion of IL‐8. C, Concentration of secreted IL‐8 measured by ELISA. The cells were pretreated with QNZ (10 nM) for 1 hour before treatment with the control (DMSO), Tp92 (5 μg/mL) or LPS (1 μg/mL) for 12 hours. *P < 005. D, NF‐κB protein expression in cells treated with Tp92 (5 μg/mL) or Tp92 (5 μg/mL)+QNZ (10 nM)