Figure 2.

CASC9 silencing impaired the proliferation, invasion in vitro, and inhibited the tumour growth in vivo. A, RT‐PCR revealed the lncRNA CASC9 expression in glioma cell lines. B, siRNAs targeted CASC9 were transfected into U87 and U251 cells to silence its expression. C, Clone formation assay illustrated the clone number in U87 and U251 cells of CASC9 silencing or controls. D, CCK‐8 assay revealed the absorbance or proliferative abilities in U87 and U251 cells of CASC9 silencing or controls. E, Transwell invasive assay illustrated the invaded cells number in U87 and U251 cells of CASC9 silencing or controls. F, In vivo xenograft mice assay illustrated the tumour volume and weight of CASC9 stable silencing by lentivirus. *P < 0.05, **P < 0.01 compared to control group