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. 2018 Sep 30;22(12):6338–6344. doi: 10.1111/jcmm.13932

Figure 3.

Figure 3

miR‐519d directly targeted the 3′‐UTR of CASC9. A, Subcellular fractionation assay revealed the subcellular location of CASC9 U87 cells. B, Complementary binding sites of miR‐519d with the 3′‐UTR of CASC9 predicted by bioinformatics online tools. C, Luciferase activity assay was conducted to confirm the binding of miR‐519d with the 3′‐UTR of CASC9. D, Ago2 RIP assay revealed the enrichment of miR‐519d and CASC9 in RNA‐induced silencing complex (RISC). E, RT‐PCR revealed the miR‐519d expression level in U87 and U251 cells. F, RT‐PCR revealed the miR‐519d expression level in U87 and U251 cells transfected with si‐CASC9. *P < 0.05, **P < 0.01 compared to control group