REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Total Rodent OXPHOS WB Antibody Cocktail	Abcam	Cat# ab110413; RRID: AB_2629281
Chemicals, Peptides, and Recombinant Proteins
Tris salt of phosphocreatine	Millipore Sigma	Cat# P1937
		CAS# 108321–17-1
Tris salt of ATP	Millipore Sigma	Cat# A9062
		CAS# 102047–34-7
Potassium pyruvate	Combi-Blocks	Cat# QA-1116
		CAS# 4151–33-1
Potassium NADP+	Ark-Pharm, Inc	Cat# AK671068
Creatine kinase from rabbit muscle	Millipore Sigma	Cat# 10736988001
Percoll solution	Millipore Sigma	Cat# P1937
		CAS#108321–17-1
Amplex Ultra Red Reagent (AUR)	ThermoFisher Scientific	Cat# A36006
CelLytic M	Millipore Sigma	Cat# C2978
Tetramethylrhodamine methyl ester (TMRM)	ThermoFisher Scientific	Cat# T668
Phosphoenoyl-pyruvate	Millipore Sigma	Cat# 10108294001;
		CAS# 4265–07-0
UK5099	Millipore Sigma	Cat# PZ0160;
		CAS# 56396–35-1
Auranofin	Millipore Sigma	Cat# A6733;
P1,P5-Di(adenosine-5ʹ)pentaphosphate	Millipore Sigma	Cat# D6392;
		CAS# 75522–97-3
Oligomycin	Millipore Sigma	Cat# 75351
		CAS# 579–13-5
Rotenone	Millipore Sigma	Cat# R8875
		CAS# 83–79-4
Potassium cyanide	Millipore Sigma	Cat# 60178
		CAS# 151–50-8
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP)	Millipore Sigma	Cat# C2920
		CAS# 370–86-5
Peroxidase from horseradish (HRP)	Millipore Sigma	Cat# P8375
		CAS# 9003–99-0
Superoxide dismutase (SOD)	Millipore Sigma	Cat# S9697
		CAS# 9054–89-1
Glucose-6-phosphate Dehydrogenase (G6PDH)	Millipore Sigma	Cat# G6378
		CAS# 9001–40-5
Malate dehydrogenase (MDH)	Millipore Sigma	Cat# 442610-M
		CAS# 9001–64-3
Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle	Millipore Sigma	Cat# P0294
Hexokinase	Millipore Sigma	Cat# H4502
		CAS# 9002–07-7
Trypsin from porcine pancreas (Trypsin)	Millipore Sigma	Cat# T4799
		CAS# 9001–51-8
Creatine Monohydrate	Millipore Sigma	Cat# C3630
		CAS# 6020–87-7
Octanoyl-L-carnitine	Millipore Sigma	Cat# 50892
		CAS# 25243–95-2
Malic acid (Malate)	Millipore Sigma	Cat# M1000
		CAS# 97–67-6
Glutamic acid (Glutamate)	Millipore Sigma	Cat# G1501
		CAS# 6382–01-0
Succinic acid (Succinate)	Millipore Sigma	Cat# S3674
		CAS# 110–15-6
α-ketoglutaric acid (AKG)	Millipore Sigma	Cat# K1750
		CAS# 328–50-7
3-Methyl-2-oxopentanoic acid (α -keto-β-methylvalerate)	Millipore Sigma	Cat#198978;
		CAS#3715–31-9
Isocitrate	Millipore Sigma	Cat#58790;
		CAS#20226–99-7
Adenosine diphosphate (ADP)	Millipore Sigma	Cat# A5285
		CAS# 72696–48-1
Tetraphenylphosphonium (TPP+)	Millipore Sigma	Cat# 218790
		CAS# 2001–45-8
NADH	Millipore Sigma	Cat# N4505
		CAS# 104809–32-7
Nicotinamide adenine dinucleotide (NAD+)	Millipore Sigma	Cat# N1636
		CAS# 53–84-9
L-Aspartic acid (Aspartate)	Millipore Sigma	Cat# A9256
		CAS# 56–84-8
Acetoacetyl-CoA	Millipore Sigma	Cat# A1625
		CAS# 1420–36-6
Coenzyme A	Millipore Sigma	Cat# C3019
		CAS# 18439–24-2
Thiamine pyrophosphate	Millipore Sigma	Cat# C8754
		CAS# 154–87-0
Pyridoxal 5′-phosphate	Millipore Sigma	Cat# P9255
		CAS# 853645–22-4
Experimental Models: Organisms/Strains
C56BL/6NJ mice	The Jackson Laboratory	Stock #005304
Software and Algorithms
Jupyter notebook containing the aΔΨ ustment calculations interspersed with procedural descriptions needed to calculate ΔGATP.	N/A	https://github.com/dmpio/bioenergeticcalculators/blob/master/jupyter_notebook/creatine-kinase-clamp.ipynb
Web-based ΔGATP calculator	N/A	https://dmpio.github.io/bioenergeticcalculators/ck_clamp/
Other
Ultracentrifuge tubes for mitochondrial percoll purifications	Beckman Coulter	Cat# 344061
Swinging bucket ultracentrifuge rotor for mitochondrial percoll purifications – Surespin 630 with tube adapters	ThermoFisher Scientific	Cat# 79363
QuantaMaster Spectrofluorometer	Horiba Scientific	Cat# QM-400