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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Pain. 2018 Dec;159(12):2620–2629. doi: 10.1097/j.pain.0000000000001373

Figure 5. ML351 and ML127 inhibit rat 15-LOX-1 activity.

Figure 5.

The inhibitors (A) ML351 and (B) ML127 were tested against rat 15-LOX-1 and 12-LOX-p. Enzymes were expressed in HEK-293T cells, incubated with vehicle or inhibitor (ML351 or ML127) for 30 min, then incubated with 70 μM arachidonic acid for 1h. The extracellular media was analyzed for 12-HETE production by LC-MS/MS. n = 4–8 wells/treatment. *P<0.05, **P<0.01, ***P<0.001.