Fig. 3.

Relative normalised expression of target genes in Ctenocephalides felis adults after immersion in double-stranded RNA (dsRNA) solutions or saline. All values were normalised to two reference genes and presented relative to the mean normalised expression level of the dsGFP treatment. (A) Relative normalised expression of GSTσ following immersion in dsGSTσ (n = 4), saline (n = 3) or dsGFP (n = 3). Treatments not sharing the same letter are significantly different (P < 0.05). (B) Relative normalised expression of Dicer-2 after immersion in dsDicer-2 (n = 4) or dsGFP (n = 6). ^*P = 0.004 which is statistically significant.