Table 1.
Sequences of oligonucleotides used in this study for both double-stranded RNA (dsRNA) cloning and quantitative PCR (qPCR). BglII (AGATCT) and XhoI (CTCGAG) restriction sites are indicated by underlined bold text.
| Target/Purpose | Primer name | Oligonucleotide sequence | Expected product size (bp) |
|---|---|---|---|
| GSTσ (dsRNA) | BglGSTσA F | AGTCAGAGATCTTATTGGAGGTCGACGGAAAG | 348 |
| XhoGSTσA R | CTAGGACTCGAGGATAACTCCGGCGAAAACAA | ||
| GSTσ (qPCR) | qGSTσA F | TGAGAACAACATGCCTACTTACAAA | 112 |
| qGSTσA R | CACTCGGACATCTTCGAATTTT | ||
| GSTσ sequencing | GSTσA F | TATTGGAGGTCGACGGAAAG | n/a |
| Dicer-2 (dsRNA) | dsDicer-2 F | CAGTTCCTCGAGATCGCAATGCTGATGGTACA | 470 |
| dsDicer-2 R | GCAGCAGATCTCAGCACAAGGAGCAAATTCA | ||
| Dicer-2 (qPCR) | qDicer-2 F | CATCTCATGGAAAGCGAAGC | 91 |
| qDicer-2 R | AAGTTGCGTAACCCGGTATG | ||
| AGO2 qPCR | qAGO2 F | CAATATGGGGGCAGAGTTTC | 143 |
| qAGO2 R | ATGGCCCACACACGTATTTC | ||
| Reference genes | Ef F | TCGTACTGGCAAATCCACAG | 145 |
| Ef R | CATGTCACGGACAACGAAAC | ||
| GAPDH F | ACCCAAAAGACTGTGGATGG | 117 | |
| GAPDH R | CGGAATGACTTTGCCTACAG |