Fig. 5.

miR-1246 targets PCBP2 and CREBL2. a PCBP2 and CREBL2 expression changes under normal and heat stress. Normal: BFR-L1 cells (lung cells) incubated at 37 °C. Heat stress: BFR-L1 cells (lung cells) incubated to temperature at 42 °C for 2 h and recovered at 37 °C for 1 h. b 3′-UTR of PCBP2 and CREBL2 were paired to the miR-1246 binding region. c Renilla/Firefly luciferase activity of candidate target genes co-transfected with mimics (miR-1246) or NC (miR-1246 negative control). d mRNA expression levels of PCBP2 and CREBL2 after cells transfected with mimics or NC. e, f Protein expression levels of PCBP2 and CREBL2 after cells transfected with mimics or NC. GAPDH was used as an internal control for mRNA and protein. Error bars represent SD (n = 3)