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. 2018 Sep 20;46(20):11061–11074. doi: 10.1093/nar/gky850

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — aIF1 discriminates a PIC assembled with an elongator-Met-tRNA from a PIC assembled with a Met-tRNA carrying three GC base pairs in the anticodon stem. Toeprinting analysis of PIC and PIC- w/o-aIF1 complexes assembled on wt-aEF1A mRNA in the presence of Met-tRNA A₁-U₇₂ (light blue), Met-tRNA elongator (green), Met-tRNA elongator 3G3C (dark blue). The toeprinting positions are indicated below the graph and to the left of the gel. Means and standard deviations are calculated from at least 3 independent experiments.